Placental transport studied by means of isolated plasma membrane vesicles
نویسندگان
چکیده
منابع مشابه
Selenium (selenate) transport by human placental brush border membrane vesicles.
1. Selenate uptake by human placental brush-border-membrane vesicles was studied in order to establish whether this anion shares a pathway with sulphate. 2. Selenate uptake was found to be saturable with respect to medium selenate and was inhibited by the anion exchange inhibitor 4,4'-diisothiocyano-stilbene-2,2'-disulphonate (DIDS). 3. Anions which have a similar tetrahedral shape to selenate,...
متن کاملSelenium (selenate) transport by human placental brush border membrane vesicles
Selenium is an essential trace element required by the fetus for normal growth, therefore knowledge on the placental transport of Se is important in the context of fetal nutrition. Recent work in this laboratory has shown that selenate is a competitive inhibitor of sulphate uptake by vesicles prepared from the brush-border membrane of the syncytiotrophoblast of the human term placenta (Shennan ...
متن کاملActive transport of 5-hydroxytryptamine by plasma membrane vesicles isolated from human blood platelets.
Plasma membrane vesicles isolated from human platelets accumulate 5-hydroxytryptamine when an electrical potential (interior negative) or an Na+ gradient (out greater than in) is imposed across the vesicle membrane. Kinetic studies reveal a Km of 0.5 micronM for the transport process. Uptake is inhibited strongly by tricyclic antidepressants and by ionophores such as gramicidin which catalyze t...
متن کاملInsertion of isolated insulin receptors into placental membrane vesicles.
Purified human insulin receptors were inserted into placental plasma-membrane vesicles by fusion of membranes with receptor-lysophosphatidylcholine micelles. Scatchard analysis of insulin binding showed that about 10-15% of the added receptors became inserted into the membrane. The receptor number could be increased about 3-fold, corresponding to approx. 5 pmol of receptor/mg of membrane protei...
متن کاملEthanolamine transport in human placental brush-border membrane vesicles.
Pathways for transport of ethanolamine by human placental epithelia were investigated by measurement of [3H]ethanolamine uptake in brush-border membrane vesicles isolated by divalent cation precipitation. The presence of a conductive uptake pathway for ethanolamine was suggested by the marked stimulation of ethanolamine uptake to levels exceeding equilibrium induced by an inside-negative potass...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Proceedings of the Nutrition Society
سال: 1991
ISSN: 0029-6651,1475-2719
DOI: 10.1079/pns19910043